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Archives 2021
Myanmar Health Sciences Research Journal
Volume 33, Number 1
TITLE: Detection of Insertion Sequence IS6110 Deficient Mycobacterium tuberculosis Strains from Myanmar
AUTHOR: Mi Mi Htwe, Wah Wah Aung , Phyu Win Ei, Aye Su Mon, Su Mon Win, Lai Lai San, Nan Aye Thida Oo,Wint Wint Nyunt & Jong Seok Lee
SOURCE: Myanmar Health Sciences Research Journal, 2021: 33,(1-3): 68-73
ABSTRACT:
Insertion sequence (IS) 6110 is a widely used target for detection of Mycobacterium tuberculosis (MTB). Molecular diagnosis of tuberculosis (TB) especially extra-pulmonary TB in Myanmar is based on detection of IS 6110 from clinical samples. However, 8-11% of MTB stains in South-East Asia do not contain this target, thus it can lead to false negative results for molecular diagnostic testing using this target. The present study was carried out to determine the occurrence of MTB complex that lacking IS6110 in Myanmar. IS6110 PCR assay was performed to detect IS6110 deficient strains among 232 MTB isolates collected during 2015-2017 from adult TB patients in different regions of Myanmar. These isolates included 125 rifampicin-resistant and 107 rifampicin-sensitive strains detected by Xpert MTB/RIF.
SUBJECT HEADINGS: IS60, Mycobacterium tuberculosis, PCR
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