Original Articles 2012-02-20

The Use of Herbal Medicine and Traditional Medicine Formulations (TMFs) for Hypertension at Traditional Medicine Hospital, Yangon

This study was done to determine the use of herbal medicines and Traditional Medicine Formulations (TMFs) for treating hypertension at Outpatients' Department (OPD) of Traditional Medicine Hospital, Yangon. This study was a cross-sectional, descriptive study in 140 hypertensive patients attending th

Original Articles 2020-09-11

Is Community Intervention Effective for Improving Maternal, Newborn and Child Health Care in Hard-to-Reach Areas of Chin State, Myanmar?

To tackle the maternal, newborn and child health (MNCH) care needs is a challenging situation in remote and hard-to-reach areas. Intervention activities focusing on strengthening community health care network through community participation and supporting Basic Health Staffs for MNCH ser

Original Articles 2020-09-14

Incidence of Snakebite in Four Townships of Yangon Region

The true incidence of snakebite in Myanmar is unknown. The current data are based on hospital returns to Ministry of Health and Sports and they are likely to be under-reported. A community-based survey is useful to know the current burden of snakebite and thus for planning and formulating str

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Aims of MHSR Journal

• To serve as an important medium for the publication of original research in the field of medical science and health research, thus filling gaps in health knowledge for effective utilization of research findings
• To impart current medical knowledge and updated scientific information obtained from research to health professionals for better and appropriate health care management
• To disseminate recent basic, applied and social research findings among health personnel of different strata for enhancing worldwide health development

Archives   2018

Myanmar   Health   Sciences   Research   Journal

Volume   30,  Number   3

TITLE:   Detection of Antinuclear Antibodies in Systemic Lupus Erythematosus Patients Using Indirect Immunofluorescence and Line Immunoassay Methods
AUTHOR:   Aye Aye Khin, Win Kalayar Kyaw & Khin Aye Thin
SOURCE:   Myanmar Health Sciences Research Journal, Vol. 30, No. 3, 2018
ABSTRACT:   Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by the production of various autoantibodies. The aim of this study was to detect antinuclear antibodies in clinically suspected SLE patients by indirect immunofluorescence (IIF) and line immunoassay (LIA) methods. Antinuclear antibodies (ANA) patterns were screened by IIF method and specific antinuclear antibodies were detected by LIA method. A laboratory-based, cross-sectional descriptive study including 30 serum samples of clinically suspected SLE patients from 500 bedded Yangon Specialty Hospital was done. ANA detected by IIF was positive in 28 (93.33%) cases and ANA by LIA was positive in 21(70%) cases. Percent agreement between the two methods was 76.7%. Four patterns of nuclear fluorescence were noted by ANA IIF method with mostly homogenous pattern (39.28%). Intermediate to good agreement (kappa=0.516) was observed between result of ANA (weak positive up to strong positive) by IIF and that by LIA method. (p=0.003). However, poor agreement (kappa=0) was observed between result of ANA (trace positive) by IIF and that by LIA method. LIA negative cases become trace positive in ANA IIF, so that ANA IIF method is more sensitive than LIA test. IIF is a gold standard to detect ANA and sensitivity of IIF was 100% and specificity was 96% according to the manufacturer's instruction. In the present study, IIF was used as a gold standard and according to calculation, sensitivity and specificity of LIA were 75% and 100%, respectively. This study suggested that LIA method can be used interchangeably with IIF method in samples from weak positive up to strong positive cases but IIF method should be used in samples negative for LIA to detect trace positivity of ANA.
SUBJECT HEADINGS:   Systemic lupus erythematosus, Antinuclear antibodies,Indirect immunofluorescence, Line immunoassay
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