Myanmar Health Sciences Research Journal
Next Prev
Aims of MHSR Journal
  • To serve as an important medium for the publication of original research in the field of medical science and health research, thus filling gaps in health knowledge for effective utilization of research findings
  • To impart current medical knowledge and updated scientific information obtained from research to health professionals for better and appropriate health care management
  • To disseminate recent basic, applied and social research findings among health personnel of different strata for enhancing worldwide health development


Archives   1993

Myanmar   Health   Sciences   Research   Journal

Volume   5,  Number   3

TITLE:   The serodiagnosis of amoebiasis by enzyme-linked immunosorbent assay.
AUTHOR:   Than Saw; Tanaka, Tomoo; Tin Tin Aye; Myint Lwin; Keneda, Yoshimasa
SOURCE:   Myanmar Health Sciences Research Journal. 1993; 5(3): 147-152
ABSTRACT:   We attempt to detect antibodies against Entamoeba histolytica in sera obtained from both healthy individuals and hospitalized patients by an enzyme-linked immunosorbent assay (ELISA). The limiting value for ELISA-positive was established on the basis of serologic results of 1869 healthy persons living in a non-endemic area (Japan) The ELISA then was applied to 61 patients in 5 hospitals in Myanmar who were suspected of having amoebiasis. The ELISA results were compared with those of stool examinations, and evaluated for sensitivity. Using the llimiting value for the ELISA, there was an 83% agreement of positivity between the ELISA tests and microscopic examinations. Specific anti-E-histolytica IgM was observed in the sera of 44 patients; IgG in all of the 61 patients. Since high titers of specific IgM were not observed in both stool-positive and stool-negative patients, we judged that patients with early stages of amoebic infections were not included in this population. In our ELISA, the sera of the patients did not exhibit crosss-reactions betweeen antigens prepared from the HK-9 strain of E. histolytica and antigens prepared from Trichomonas vaginalis and Giardia lamblia. On the basis of these results, we suggest that it is necessary to do two or more different examinations in order to make a definite diagnosis of amoebic infection.
SUBJECT HEADINGS:   Enzyme-Linked Immunosorbent Assay. Serologic Tests. Amebiasis
FULL TEXT:  

Back to Articles

Vision : Achieving a healthier nation through application of research findings          Mission Statement : To Develop and promote solutions to the major health problems of Myanmar