Myanmar Health Sciences Research Journal
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Aims of MHSR Journal
  • To serve as an important medium for the publication of original research in the field of medical science and health research, thus filling gaps in health knowledge for effective utilization of research findings
  • To impart current medical knowledge and updated scientific information obtained from research to health professionals for better and appropriate health care management
  • To disseminate recent basic, applied and social research findings among health personnel of different strata for enhancing worldwide health development


Archives   2005

Myanmar   Health   Sciences   Research   Journal

Volume   17,  Number   2

TITLE:   Assessment of whole cell soluble mycobacterium tuberculosis antigen ELISA (in-house ELISA) test in serodiagnosis of pulmonary tuberculosis.
AUTHOR:   Khin Nwe Oo; Nwe Nwe Yin; Tin Tin Han; Myat Thida; Wah Wah Aung
SOURCE:   Myanmar Health Sciences Research Journal. 2005; 17(2): 116-117
ABSTRACT:   A rapid, reliable and inexpensive serological test for diagnosis of tuberculosis would have great applicability as a clinical, epidemio-logical and public health tool. Therefore, use of whole cell soluble Mycobacterium tuberculosis antigen ELISA (in-house test) as a serodiagnostic test for pulmonary tuberculosis was studied. This study was carried out from February 2002 to January 2003. It was a cross-sectional, laboratory and institutional-based study. Coating antigen was prepared in Tuberculosis Culture Laboratory and Immu-nology Research Division, Department of Medical Research (Lower Myanmar). It was prepared from Mycobacterium tuberculosis cultured on 3% Ogawa media. Whole cell soluble M. tuberculosis antigen was prepared using the method of Nicholl's (1975). Phenolized non-viable tubercle bacilli were collected, treated with N sodium hydroxide and heated at 80°C for 30 minutes. After washing and centrifuging, antigen soluble in double distilled water was used as coating antigen in ELISA test.
SUBJECT HEADINGS:   Mycobacterium tuberculosis. Enzyme-Linked Immunosorbent Assay. Tuberculosis, Pulmonary. Serologic Tests.
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Vision : Achieving a healthier nation through application of research findings          Mission Statement : To Develop and promote solutions to the major health problems of Myanmar