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Archives 2005
Myanmar Health Sciences Research Journal
Volume 17, Number 2
TITLE: Diagnosis of plasmodium falciparum infection from two different endemic areas by two immunological methods using glycophospholipid antigen.
AUTHOR: Maung Maung Mya; Saxena, R.K; A.Roy; Roy, K.B; W.Tun Lin; Sein Min; Sein Thaung; Sein Maung Than; Kyaw Zaw
SOURCE: Myanmar Health Sciences Research Journal. 2005; 17(2): 55-61
ABSTRACT: Previously described isolated Glycophospholipid (GPL) antigen's diagnostic sensitivity and specificity properties were tested on malarial and non malarial blood samples from two different malaria endemic areas in Myanmar and India. The control area is a non-endemic area, Delhi, India. Standard Enzyme-Link Immunosorbent Assay (ELISA) and modified Laser Immunoassay (LIA) methods were used. The crude parasite rates were found to be 28.75% in Assam, India and 32.5% in Taikkyi, Myanmar respectively. P. falciparum was predominantly high in both forested-hilly areas. Immunoreactivity and specificity of GPL antigen were compared with those of another previously reported RESA derived synthetic peptide antigen (R1). The diagnostic sensitivity properties of GPL antigen were found cent percent sensitive for P. falciparum cases in both areas by LIA method but ELISA with R1 antigen showed a slightly lower level of sensitivity than GPL antigen. The specificity studies of GPL and R1 antigens tested on malarial and non malarial diseases sera were found to be very specific for P. falciparum as they did not react with other non malarial diseases sera. LIA method with the application of GPL coated latex beads can be an excellent diagnostic tool for Plasmodium falciparum infection.
SUBJECT HEADINGS: Plasmodium falciparum. Antigens.
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