- To serve as an important medium for the publication of original research in the field of medical science and health research, thus filling gaps in health knowledge for effective utilization of research findings
- To impart current medical knowledge and updated scientific information obtained from research to health professionals for better and appropriate health care management
- To disseminate recent basic, applied and social research findings among health personnel of different strata for enhancing worldwide health development
Archives 2006
Myanmar Health Sciences Research Journal
Volume 18, Number 2
TITLE: Antibacterial activity of oils of clove and garlic on bacterial isolates (special emphasis on coagulase-positive Staphylococcus aureus) from patients with septic wound (anapauk) from Traditional Medicine Hospital.
AUTHOR: Lwin Lwin Cho; Mar Mar Nyein; Win Myint; Sein Win; May Kyi Aung
SOURCE: Myanmar Health Sciences Research Journal. 2006; 18(2): 78-83
ABSTRACT: Wound swab samples from 50 patients (age ranged from 13-69 years) attending Traditional Medicine Hospital from 25 January to 26 April 2005, were collected in Stuart's transport media. Isolation of bacterial pathogens was proceeded by standard methods, using Blood agar, Mannitol Salt agar, MacConkey agar, Nutrient agar and Ashdown agar. Further diagnosis was done by Gram staining, biochemical and serology reaction. Among them, coagulase - positive Staphylococcus aureus was isolated from 30 cases (60%). Moreover, Escherichia coli from8 cases (16%), Klebsiella aerogenes from 3 cases (6.0%), Proteus vulgaris from 6 cases (12.0%) and Pseudomonas pyocyanea from one case (2.0%) were also identified. The antibiogram of Staphylococcus aureus revealed that 96.7% were susceptible to sulfamethoxazole-trimethoprim followed by tetracycline (53.3%), cefotaxime (13.3%) and erythromycin (3.3%). It was found that 18, 27, 15, 20, 20, 30 and 24 isolates were susceptible to tetracycline, ceftriazone, cefalothin, erythromycin, cloxacillin, clove oil and garlic oilrespectively. Majority of them were intermediate susceptible to ampicillin (70.0%) and resistant to cloxacillin (23.3%).The extracts of three medicinal plants: Allium sativum (kyet-thun-phyu) bulb, Azadirachta indica (tamar) leaves and Eupatorium odorantum (bizat) leaves by 97% ethanol, 50% ethanol and watery types were tested on the isolated S. aureusbut showed no anti-bacterial activity. However, the antibacterial activity was obtained when tested with fresh juice of garlic and clove oil. The Minimum Inhibitory Concentration (MIC) of fresh garlic juice was 1.3 to 2% (v/v) and 1.3 to 5% (v/v) by agar plate dilution and test tube serial dilution methods respectively.
SUBJECT HEADINGS: Plants, Medicinal. Garlic. Eugenol.
FULL TEXT: