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Aims of MHSR Journal

• To serve as an important medium for the publication of original research in the field of medical science and health research, thus filling gaps in health knowledge for effective utilization of research findings
• To impart current medical knowledge and updated scientific information obtained from research to health professionals for better and appropriate health care management
• To disseminate recent basic, applied and social research findings among health personnel of different strata for enhancing worldwide health development

Archives   2006

Myanmar   Health   Sciences   Research   Journal

Volume   18,  Number   1

TITLE:   Antibacterial activity of some plants and formulations and determination of Minimum Inhibitory Concentration (MIC) by microtitre plate dilution method.
AUTHOR:   Mar Mar Nyein; Mi Mi Htwe; Ba Han; Ei Ei Khine
SOURCE:   Myanmar Health Sciences Research Journal. 2006; 18(1): 26-30
ABSTRACT:   Traditional Medicine Formulation (TMF-01) [mixture of Cyperus rotundus Linn. (_rufrkefnif;),Alpinia galangalWalld(y'JaumjuD;) and Piper betle Linn.(uGrf;)] and plants [Alpinia galangal Walld. (y'JaumjuD;)Acorus calamus Linn.(vif;ae), and Piper longum Linn. (ydwfcsif;)] were tested for their antibacterial activity on 20 different types of bacteria (Escherichia coli=6 types, Proteus morganii, Shigellaboydii, S. dysenteriae, S. flexneri,S. sonnei, S. derby, S. krefeld, Staphylococcus aureus; S. epidermidis, Vibrio cholerae O1,V. cholerae O139, V. cholerae Inaba,V. cholerae Ogawa and V. fluvialis) from clinical sources. The bacteriostatic and bactericidal activities of extracts were tested by microtitre plate dilution method and the optical density was determined by microplate reader. It was found that water extract of TMF had antibacterial activity on 20 different types of bacteria with various inhibition zone sizes ranging from 14 to 26 mm. Similarly, water extract of ash and water extract of ash from water extract of Alpinia galangal Walld (y'JaumjuD;) possess antibacterial activity with the zone size of 7-18 mm. The asarone and methyl piperate compound obtained from Acorus calamus Linn. (vif;ae), and Piper longumLinn.(ydwfcsif;)respectively also showed antibacterial activity on E. coli,S. flexneri, S. aureusand P. aeruginosa.The Minimum Inhibitory Concentration (MIC) of water extracts of TMF -01 was ranging from 0.16 to 0.32mg/ml. Similarly, the MIC of water extract of ash of Alpinia galangal Walld(y'JaumjuD;) was 0.16 mg/ml and water extract of ash from water extract of Alpinia galangal Walld(y'JaumjuD;) ranged from 0.078 to 0.625mg/ml. Moreover, the MIC of asarone from Acorus calamus Linn. (vif;ae) was 0.06-0.12mg/ml and that of methyl piperate from Piper longumLinn.(ydwfcsif;) was 0.03mg/ml.
SUBJECT HEADINGS:   Attention. Plants, Medicinal.
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