Original Articles 2012-02-20

The Use of Herbal Medicine and Traditional Medicine Formulations (TMFs) for Hypertension at Traditional Medicine Hospital, Yangon

This study was done to determine the use of herbal medicines and Traditional Medicine Formulations (TMFs) for treating hypertension at Outpatients' Department (OPD) of Traditional Medicine Hospital, Yangon. This study was a cross-sectional, descriptive study in 140 hypertensive patients attending th

Original Articles 2020-09-11

Is Community Intervention Effective for Improving Maternal, Newborn and Child Health Care in Hard-to-Reach Areas of Chin State, Myanmar?

To tackle the maternal, newborn and child health (MNCH) care needs is a challenging situation in remote and hard-to-reach areas. Intervention activities focusing on strengthening community health care network through community participation and supporting Basic Health Staffs for MNCH ser

Original Articles 2020-09-14

Incidence of Snakebite in Four Townships of Yangon Region

The true incidence of snakebite in Myanmar is unknown. The current data are based on hospital returns to Ministry of Health and Sports and they are likely to be under-reported. A community-based survey is useful to know the current burden of snakebite and thus for planning and formulating str

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Aims of MHSR Journal

• To serve as an important medium for the publication of original research in the field of medical science and health research, thus filling gaps in health knowledge for effective utilization of research findings
• To impart current medical knowledge and updated scientific information obtained from research to health professionals for better and appropriate health care management
• To disseminate recent basic, applied and social research findings among health personnel of different strata for enhancing worldwide health development

Archives   2006

Myanmar   Health   Sciences   Research   Journal

Volume   18,  Number   1

TITLE:   Evaluation of Polymerase Chain Reaction (PCR) Amplification of Mycobacterium leprae in biopsy specimens from leprosy patients.
AUTHOR:   Khin Saw Aye; Yin Min Htun; Aye Aye Win; Tin Zar Maw; Kyaw Kyaw
SOURCE:   Myanmar Health Sciences Research Journal. 2006; 18(1): 21-25
ABSTRACT:   Eighty two skin biopsy specimens from leprosy patients attending at Central Special Skin Clinic, Yangon General Hospital were collected before taking chemotherapy. They were examined by a two-step polymerase chain reaction assay using a set of four nested oligonucleotide primers for the detection and identification of Mycobacterium leprae. It did not require the use of radioactively labelled hybridization probes. The nested-primer procedure amplified a 347base-pair product from M. leprae genomic DNA. The PCR results were then compared with bacterial indices (BI) of slit-skin smears. PCR was positive in 8 (80%) of 10 biopsy specimens with BI of 0 determined for the slit-skin smear from the same patients. PCR also gave positive results for 71 (98.6%) of 72 BI positive cases. The agreement rate of these two tests is 89% but the false negative rate of BI for diagnosis of leprosy compared to PCR method is 10.39%. According to these results, PCR has an advantage over microscopic examination in detecting M. leprae in biopsy specimens negative for acid-fast bacilli, and is a useful tool for laboratory diagnosis.
SUBJECT HEADINGS:   Polymerase Chain Reaction. Mycobacterium leprae. Biopsy. Leprosy.
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