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Archives 2006
Myanmar Health Sciences Research Journal
Volume 18, Number 1
TITLE: Isolation of anaerobic Gram-positive bacilli and detection of Clostridium perfringens alpha toxin from some foods.
AUTHOR: Thin Thin Maw; Mar Mar Nyein; Mi Mi Htwe; Aye Aye Maw
SOURCE: Myanmar Health Sciences Research Journal. 2006; 18(1): 13-16
ABSTRACT: Isolation of anaerobic Gram-positive bacilli and detection of Clostridium perfringensalpha toxin were done on 120 samples of different food collected from shops available around Yangon area during 2003. After culturing in Trypticase Glucose Yeast (TGY) media, Clostridium perfringens alpha toxin was detected by using ELISA kit obtained from Bio-X Diagnostics, Belgium. Anaerobic Gram- positive bacilli was isolated from 31 samples (25.83%). They were isolated from 16.67%, 25%, 29.17%, 20.83% and 37.5% of noodles, fermented vegetables, fermented fish and prawns, salted fish paste (ngapi) and pickled tea respectively. The toxins were detected from 20 samples of food: 4.17% (1/24 samples of noodles), 45.80% (11/24 samples of fermented vegetables such as salted/fermented beans) and 4.17% (1/24 samples of fermented fish & prawns), 29.19% (7/24 from ngapi) respectively. Thus, this study indicates that toxin could be elaborated in protein rich preserved foods (fish / prawn paste).
SUBJECT HEADINGS: Gram-Positive Bacteria. Clostridium perfringens. Phospholipase C. Food Microbiology.
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