Original Articles 2012-02-20

The Use of Herbal Medicine and Traditional Medicine Formulations (TMFs) for Hypertension at Traditional Medicine Hospital, Yangon

This study was done to determine the use of herbal medicines and Traditional Medicine Formulations (TMFs) for treating hypertension at Outpatients' Department (OPD) of Traditional Medicine Hospital, Yangon. This study was a cross-sectional, descriptive study in 140 hypertensive patients attending th

Original Articles 2020-09-11

Is Community Intervention Effective for Improving Maternal, Newborn and Child Health Care in Hard-to-Reach Areas of Chin State, Myanmar?

To tackle the maternal, newborn and child health (MNCH) care needs is a challenging situation in remote and hard-to-reach areas. Intervention activities focusing on strengthening community health care network through community participation and supporting Basic Health Staffs for MNCH ser

Original Articles 2020-09-14

Incidence of Snakebite in Four Townships of Yangon Region

The true incidence of snakebite in Myanmar is unknown. The current data are based on hospital returns to Ministry of Health and Sports and they are likely to be under-reported. A community-based survey is useful to know the current burden of snakebite and thus for planning and formulating str

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Aims of MHSR Journal

• To serve as an important medium for the publication of original research in the field of medical science and health research, thus filling gaps in health knowledge for effective utilization of research findings
• To impart current medical knowledge and updated scientific information obtained from research to health professionals for better and appropriate health care management
• To disseminate recent basic, applied and social research findings among health personnel of different strata for enhancing worldwide health development

Archives   2007

Myanmar   Health   Sciences   Research   Journal

Volume   19,  Number   3

TITLE:   Detection of low level viral genome (HBV DNA) by Polymerase Chain Reaction in nonresponders of plasma-derived hepatitis B vaccine produced at the Department of Medical Research (Lower Myanmar).
AUTHOR:   Moh Moh Htun; Khin Pyone Kyi; Khin May Oo; San San Oo; Khin May Oo; Ohnmar Lwin; Kenji Abe
SOURCE:   Myanmar Health Sciences Research Journal. 2007; 19(3): 125-130
ABSTRACT:   Myanmar is an area of high endemicity for hepatitis B with a 35-60% infection rate and 10-15% HBsAg carrier rate. Several vaccine trials carried out at the Department of Medical Research (Lower Myanmar) using plasma- derived hepatitis B vaccine have shown that there was approximately 10% non-responder rate. Out of 337 apparently healthy persons who were initially screened for HBsAg, antiHBs and antiHBc by using the Abbott ELISA test kits (USA), 137 persons were negative for all markers (HBsAg, AntiHBs and AntiHBc). They were immunized with three doses of locally produced plasma-derived hepatitis B vaccine (10μg/ml) by intramuscular route in the deltoid region. One month after the completion of three doses of hepatitis B vaccine, 38 subjects who did not respond were vaccinated again using the same dose, site and route. Out of 38 vaccinees, 23 subjects did not have detectable levels (10 m IU/L) at two months after the booster vaccination. HBV DNA was extracted from all serum samples of nonresponders by using the phenol, chloroform and ethanol method. These extracted samples were tested for HBV DNA by using the Polymerase Chain Reaction (PCR). After the amplification of 40 cycles, HBV DNA was detected by 2% agarose gel electrophoresis. The short nucleotide of the HBV DNA (< 200bp) of the positive two samples were amplified successfully by nested PCR and sequenced by Taq Dye Deoxy TM Terminator Cycle Sequencing Kit. These two nonresponders (8.69%) may be due to silent HBV infection.
SUBJECT HEADINGS:   Genomics. Polymerase Chain Reaction. Hepatitis B Vaccines.
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