Original Articles 2012-02-20

The Use of Herbal Medicine and Traditional Medicine Formulations (TMFs) for Hypertension at Traditional Medicine Hospital, Yangon

This study was done to determine the use of herbal medicines and Traditional Medicine Formulations (TMFs) for treating hypertension at Outpatients' Department (OPD) of Traditional Medicine Hospital, Yangon. This study was a cross-sectional, descriptive study in 140 hypertensive patients attending th

Original Articles 2020-09-11

Is Community Intervention Effective for Improving Maternal, Newborn and Child Health Care in Hard-to-Reach Areas of Chin State, Myanmar?

To tackle the maternal, newborn and child health (MNCH) care needs is a challenging situation in remote and hard-to-reach areas. Intervention activities focusing on strengthening community health care network through community participation and supporting Basic Health Staffs for MNCH ser

Original Articles 2020-09-14

Incidence of Snakebite in Four Townships of Yangon Region

The true incidence of snakebite in Myanmar is unknown. The current data are based on hospital returns to Ministry of Health and Sports and they are likely to be under-reported. A community-based survey is useful to know the current burden of snakebite and thus for planning and formulating str

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Aims of MHSR Journal

• To serve as an important medium for the publication of original research in the field of medical science and health research, thus filling gaps in health knowledge for effective utilization of research findings
• To impart current medical knowledge and updated scientific information obtained from research to health professionals for better and appropriate health care management
• To disseminate recent basic, applied and social research findings among health personnel of different strata for enhancing worldwide health development

Archives   2007

Myanmar   Health   Sciences   Research   Journal

Volume   19,  Number   3

TITLE:   Stability of the recombinant hepatitis B surface antigen expression vector in genomic DNA of Hansenula polymorpha yeast cells (Master Cell Bank).
AUTHOR:   Win Aung; Suk Hoon Ha; Jae Seung Kim; Yeon Hee Kim; Khin Pyone Kyi
SOURCE:   Myanmar Health Sciences Research Journal. 2007; 19(3): 121-124
ABSTRACT:   For productionof recombinanthepatitis B (HB) vaccine, fermentation of the transformed Hansenula polymorpha yeast cells containing hepatitis B surface antigen (HBs Ag) expression vector stored as lyophilizedform (Master Cell Bank) at 4ºC followed by several purification steps has to be performed. In our study, the existence of the structural HBsAg gene and URA 3 selective marker gene in the HBsAg expression vector, integrated in chromosomal DNA of recombinant Hansenula polymorpha yeast cell was determined. First, genomic DNA of Hansenula polymorpha yeast cell was extracted from MCB and purified by using QIAGEN genomic DNA extracting kit, followed by restriction enzyme digestion, PCR amplification, agarose gel electrophoresis and Southern blot hybridization. The nucleotide sequence of HBsAg DNA segment was also confirmed by DNA Genetic Analyser. In addition, the presence of the URA3 marker gene in the HBsAg expression vector was also identified by culturing of transformed Hansenula polymorpha colonies on the FOA containing culture plate for their survival.The results indicated that both structural HBsAg expressed gene and URA 3 marker gene were found to be stable in chromosomal DNA of Hansenula polymorpha yeast cells at 4ºC on long-term storage.
SUBJECT HEADINGS:   Drug Stability. Hepatitis B Surface Antigens. DNA. Genomics.
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