Original Articles 2012-02-20

The Use of Herbal Medicine and Traditional Medicine Formulations (TMFs) for Hypertension at Traditional Medicine Hospital, Yangon

This study was done to determine the use of herbal medicines and Traditional Medicine Formulations (TMFs) for treating hypertension at Outpatients' Department (OPD) of Traditional Medicine Hospital, Yangon. This study was a cross-sectional, descriptive study in 140 hypertensive patients attending th

Original Articles 2020-09-11

Is Community Intervention Effective for Improving Maternal, Newborn and Child Health Care in Hard-to-Reach Areas of Chin State, Myanmar?

To tackle the maternal, newborn and child health (MNCH) care needs is a challenging situation in remote and hard-to-reach areas. Intervention activities focusing on strengthening community health care network through community participation and supporting Basic Health Staffs for MNCH ser

Original Articles 2020-09-14

Incidence of Snakebite in Four Townships of Yangon Region

The true incidence of snakebite in Myanmar is unknown. The current data are based on hospital returns to Ministry of Health and Sports and they are likely to be under-reported. A community-based survey is useful to know the current burden of snakebite and thus for planning and formulating str

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Aims of MHSR Journal

• To serve as an important medium for the publication of original research in the field of medical science and health research, thus filling gaps in health knowledge for effective utilization of research findings
• To impart current medical knowledge and updated scientific information obtained from research to health professionals for better and appropriate health care management
• To disseminate recent basic, applied and social research findings among health personnel of different strata for enhancing worldwide health development

Archives   2007

Myanmar   Health   Sciences   Research   Journal

Volume   19,  Number   2

TITLE:   Viability of the recombinant hepatitis B surface antigen expressed-Hansenula polymorpha yeast cells in Master Cell Bank.
AUTHOR:   Win Aung; Jae Seung Kim; Nam Joong Le; Yeon Hee Kim; Khin Phone Kyi
SOURCE:   Myanmar Health Sciences Research Journal. 2007; 19(2): 94-96
ABSTRACT:   For production of the recombinant hepatitis B ( HB ) vaccine, freeze-dried form of Master Cell Bank(MCB ) containing the HBsAg expressed- Hansenula polymorpha yeast cells , stored at 4ºC has been used as a starting material, followed by seed cultivation, fermentation and purification processes. In this study, freeze-dried form of MCB was reconstituted and viability of the HBsAg expressed-Hansenula polymorpha yeast cells was determined by observing cell morphology, growth pattern and presence of contamination during cultivation. It was found that there were actively growing viable yeast cells at different stages of cell divisions with specific characteristics. These cells also showed an increase in growth during cultivation with normal pattern of growth curve and were free from any contamination. Therefore, the recombinant hepatitis B surface antigen expressed -Hansenula polymorpha yeast cells in long-term storage at 4ºC in MCB were found to be viable, stable and safe satisfactorilythus being suitable for further processing recombinant HB vaccine production.
SUBJECT HEADINGS:   Fetal Viability. Hepatitis B Surface Antigens.
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