Original Articles 2012-02-20

The Use of Herbal Medicine and Traditional Medicine Formulations (TMFs) for Hypertension at Traditional Medicine Hospital, Yangon

This study was done to determine the use of herbal medicines and Traditional Medicine Formulations (TMFs) for treating hypertension at Outpatients' Department (OPD) of Traditional Medicine Hospital, Yangon. This study was a cross-sectional, descriptive study in 140 hypertensive patients attending th

Original Articles 2020-09-11

Is Community Intervention Effective for Improving Maternal, Newborn and Child Health Care in Hard-to-Reach Areas of Chin State, Myanmar?

To tackle the maternal, newborn and child health (MNCH) care needs is a challenging situation in remote and hard-to-reach areas. Intervention activities focusing on strengthening community health care network through community participation and supporting Basic Health Staffs for MNCH ser

Original Articles 2020-09-14

Incidence of Snakebite in Four Townships of Yangon Region

The true incidence of snakebite in Myanmar is unknown. The current data are based on hospital returns to Ministry of Health and Sports and they are likely to be under-reported. A community-based survey is useful to know the current burden of snakebite and thus for planning and formulating str

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Aims of MHSR Journal

• To serve as an important medium for the publication of original research in the field of medical science and health research, thus filling gaps in health knowledge for effective utilization of research findings
• To impart current medical knowledge and updated scientific information obtained from research to health professionals for better and appropriate health care management
• To disseminate recent basic, applied and social research findings among health personnel of different strata for enhancing worldwide health development

Archives   2007

Myanmar   Health   Sciences   Research   Journal

Volume   19,  Number   2

TITLE:   Optimization of a multiplex polymerase chain reaction assay and its application for simultaneous detection of Neisseria gonorrhoeae and Chlamydia trachomatis inclinical specimens.
AUTHOR:   Wah Wah Aung; Mar Mar Nyein; Khin May Oo; Khin Nwe Oo; Tiang Yee Peng; Ngeow Yun Fong
SOURCE:   Myanmar Health Sciences Research Journal. 2007; 19(2): 81-86
ABSTRACT:   Chlamydia trachomatis and Neisseria gonorrhoeae infections are common sexually transmitted infections with a similar pattern of clinical presentation and related sequelae such as urethritis, cervicitis, pelvic inflammatory disease, ectopic pregnancy and infertility. Chlamydial co-infections are also frequently found in patients with gonorrhoea. This study was conducted to optimize a multiplex polymerase chain reaction (M-PCR) assay which could be applied to detect N. gonorrhoeae and C. trachomatis simulta-neously from genitourinary specimens. The procedure of a M-PCR assay, developed by Mahony et al. (1995), was modified by incorporating lambda phage-Neisseria hybrid DNA as an internal control for the detection of amplification inhibitors. Thermal cycling parameters were optimized by varying annealing temperatures and using different concentrations of magnesium chloride, dNTPs and primers. The optimized M-PCR assay had a detection limit of 2.885 x 103 genomecopies for C. trachomatis and 8.995x102 genome copies for N. gonorrhoeae. It was used to detect N. gonorrhoeae and C. trachomatis in 90 endocervical swab specimens from women with vaginal discharge. The specimens were also tested for chlamydial antigen using Clear View test kit (Unipath, UK) and for N. gonorrhoeae by conventional culture. The sensitivity and specificity of the M-PCR assay were found to be superior to those of gonococcal culture and chlamydia antigen testing, making it a potentially useful test for the diagnosis of N. gonorrhoeae and C. trachomatis in lower genital tract infections in symptomatic women.
SUBJECT HEADINGS:   Polymerase Chain Reaction. Neisseria gonorrhoeae. Chlamydia trachomatis.
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