- To serve as an important medium for the publication of original research in the field of medical science and health research, thus filling gaps in health knowledge for effective utilization of research findings
- To impart current medical knowledge and updated scientific information obtained from research to health professionals for better and appropriate health care management
- To disseminate recent basic, applied and social research findings among health personnel of different strata for enhancing worldwide health development
Archives 2007
Myanmar Health Sciences Research Journal
Volume 19, Number 1
TITLE: Use of in-house test system for investigating of Plasmodium falciparum antisporozoite antibodies in a malaria endemic area.
AUTHOR: Muya Than; Aye Aye Myint; Aye Aye Maw; Sandar Aung; Myo Khin; Ye Htut; Khin May Oo; Aye Aye Yee; Yi Yi Kyaw
SOURCE: Myanmar Health Sciences Research Journal. 2007; 19(1): 53-56
ABSTRACT: Malaria ranks as first priority disease in Myanmar. For effective control of malaria, the situation of malaria transmission in the area must be known as much as possible. Of several indicators, antisporozoite antibody is considered as a useful indicator for assessing the transmission and could be used in planning the progress of vector control programmes. We developed an in-house Indirect Enzyme Immunosorbent Assay (EIA) system using synthetic peptide NANP3 as the solid phase and peroxidase labeled anti-human IgG (Rabbit) as the conjugate. Checkerboard titration was carried out and the dilutions determined. The antisporozoite antibody levels of 384 subjects from Tarchileik (mean age 34.71 ± 19.2yrs) were determined. The mean antibody levels ranged from 0.08µg to 21.9µg. No significant difference in the anti-sporozoite antibody positive rate was found between males and females (29.2% vs 37.6%). A positive correlation (r = 0.32) with age (p<0.001) was found and highest antisporozoite antibody positive rate was found in 20-40 years age group followed by 40-60 years age group. No association was found with history of malaria. The developed EIA could be used to assess the degree of malaria transmission in a locality.
SUBJECT HEADINGS: Plasmodium falciparum. Malaria.
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