Original Articles 2012-02-20

The Use of Herbal Medicine and Traditional Medicine Formulations (TMFs) for Hypertension at Traditional Medicine Hospital, Yangon

This study was done to determine the use of herbal medicines and Traditional Medicine Formulations (TMFs) for treating hypertension at Outpatients' Department (OPD) of Traditional Medicine Hospital, Yangon. This study was a cross-sectional, descriptive study in 140 hypertensive patients attending th

Original Articles 2020-09-11

Is Community Intervention Effective for Improving Maternal, Newborn and Child Health Care in Hard-to-Reach Areas of Chin State, Myanmar?

To tackle the maternal, newborn and child health (MNCH) care needs is a challenging situation in remote and hard-to-reach areas. Intervention activities focusing on strengthening community health care network through community participation and supporting Basic Health Staffs for MNCH ser

Original Articles 2020-09-14

Incidence of Snakebite in Four Townships of Yangon Region

The true incidence of snakebite in Myanmar is unknown. The current data are based on hospital returns to Ministry of Health and Sports and they are likely to be under-reported. A community-based survey is useful to know the current burden of snakebite and thus for planning and formulating str

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Aims of MHSR Journal

• To serve as an important medium for the publication of original research in the field of medical science and health research, thus filling gaps in health knowledge for effective utilization of research findings
• To impart current medical knowledge and updated scientific information obtained from research to health professionals for better and appropriate health care management
• To disseminate recent basic, applied and social research findings among health personnel of different strata for enhancing worldwide health development

Archives   2007

Myanmar   Health   Sciences   Research   Journal

Volume   19,  Number   1

TITLE:   Establishment of a simple procedure to detect the yield of recombinant HBsAg protein expressed by transformed Hansenula polymorpha Yeast cells (MCB).
AUTHOR:   Win Aung; Hyunah Kim; Yeon Hee Kim; Suk Hoon Ha; Khi Pyone Kyi
SOURCE:   Myanmar Health Sciences Research Journal. 2007; 19(1): 32-36
ABSTRACT:   For large scale production of recombinant Hepatitis B vaccine, recombinant Hansenula polymorpha yeast cells containing the HBsAg -expressed gene ( Master Cell Bank ) was processed under cultivation in seed and main fermentors ,followed by cell disruption and purificationby using concentrator, homogenizer, ultracentrifuge, column chromatography , and sterilization filtration to have a purified HBsAgbulk. The whole process takes about 2 months. In our study, a single colony of MCB strain was cultured in the simple culture tube with methanol feeding followed by glass bead disruption of recombinant yeast cells. The released HBsAg protein was determined by using the AUSZYME test kit. The identity and purity of recombinant HBsAg protein was confirmed by SDS PAGE and Western blot hybridization. The whole procedure took about one week. It also indicates the viability of recombinant Hansenula polymorpha yeast cells and stability of HBsAg - expressed gene in the MCB. This is a simple and rapid procedure to predict the yield of HBsAg protein expressed by recombinant Hansenula polymorpha yeast cells prior to the actual production process of recombinant HB vaccine in the Plant.
SUBJECT HEADINGS:   Hepatitis B Surface Antigens.
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