Original Articles 2012-02-20

The Use of Herbal Medicine and Traditional Medicine Formulations (TMFs) for Hypertension at Traditional Medicine Hospital, Yangon

This study was done to determine the use of herbal medicines and Traditional Medicine Formulations (TMFs) for treating hypertension at Outpatients' Department (OPD) of Traditional Medicine Hospital, Yangon. This study was a cross-sectional, descriptive study in 140 hypertensive patients attending th

Original Articles 2020-09-11

Is Community Intervention Effective for Improving Maternal, Newborn and Child Health Care in Hard-to-Reach Areas of Chin State, Myanmar?

To tackle the maternal, newborn and child health (MNCH) care needs is a challenging situation in remote and hard-to-reach areas. Intervention activities focusing on strengthening community health care network through community participation and supporting Basic Health Staffs for MNCH ser

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Incidence of Snakebite in Four Townships of Yangon Region

The true incidence of snakebite in Myanmar is unknown. The current data are based on hospital returns to Ministry of Health and Sports and they are likely to be under-reported. A community-based survey is useful to know the current burden of snakebite and thus for planning and formulating str

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Aims of MHSR Journal

• To serve as an important medium for the publication of original research in the field of medical science and health research, thus filling gaps in health knowledge for effective utilization of research findings
• To impart current medical knowledge and updated scientific information obtained from research to health professionals for better and appropriate health care management
• To disseminate recent basic, applied and social research findings among health personnel of different strata for enhancing worldwide health development

Archives   2008

Myanmar   Health   Sciences   Research   Journal

Volume   20,  Number   3

TITLE:   Identification of host-derived Hansenula polymorpha DNA in the recombinant hepatitis B vaccine by using a dot-blot hybridization method.
AUTHOR:   Win Aung; Nam Joong Lee; Yeon Hee Kim; Suk Hoon Ha; Khin Pyone Kyi
SOURCE:   Myanmar Health Sciences Research Journal. 2008; 20(3): 147-151
ABSTRACT:   In the recombinant hepatitis B (HB)vaccine produced by using yeast as an host organism, the residue of the host-derived DNA in more than permissible amount can cause undesirable effect on vaccine recipients. In this study, the host-derived Hansenula polymorphaDeoxyribonucleic Acid (DNA)in the recombinant hepatitis B vaccine produced by CJ Pharmaceutical Plant, Republic of Koreawas identified by using a dot blot hybridization method. First, a single strand hybridization probe was prepared by a template extracted from Hansenula polymorphaDNA and was labeled with d-UTP-DIG by using PCR technique followed by testing of the labeling efficacy of the probe. The host-derived DNA fromrecombinant HB vaccinesamples was extracted by usingphenol/chloroform method to obtain DNA pellets whereas standard DNA with known concentration was prepared in different dilutions. Then hybridization of vaccine samples and standards was done on hybridization nylon membrane by using a prepared hybridization probe labeled with dUTP-DIG in the UV cross linker,hybridization oven,sealable plastic bag and several kinds of washing, detecting and developing solutions/buffers. Finally, DNA contents in vaccine samples were quantified on hybridization membrane by comparing the intensity of specific color signals with those of the standard DNA in different concentrations.No apparent color signal was observed in all 3 test samples of recombinant HB vaccine thus indicating the total absence of host-derived DNA in recombinant HB vaccine and total safety of it for human use.
SUBJECT HEADINGS:   Pichia. DNA. Hepatitis B Vaccines. Hybridization.
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