Original Articles 2012-02-20

The Use of Herbal Medicine and Traditional Medicine Formulations (TMFs) for Hypertension at Traditional Medicine Hospital, Yangon

This study was done to determine the use of herbal medicines and Traditional Medicine Formulations (TMFs) for treating hypertension at Outpatients' Department (OPD) of Traditional Medicine Hospital, Yangon. This study was a cross-sectional, descriptive study in 140 hypertensive patients attending th

Original Articles 2020-09-11

Is Community Intervention Effective for Improving Maternal, Newborn and Child Health Care in Hard-to-Reach Areas of Chin State, Myanmar?

To tackle the maternal, newborn and child health (MNCH) care needs is a challenging situation in remote and hard-to-reach areas. Intervention activities focusing on strengthening community health care network through community participation and supporting Basic Health Staffs for MNCH ser

Original Articles 2020-09-14

Incidence of Snakebite in Four Townships of Yangon Region

The true incidence of snakebite in Myanmar is unknown. The current data are based on hospital returns to Ministry of Health and Sports and they are likely to be under-reported. A community-based survey is useful to know the current burden of snakebite and thus for planning and formulating str

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Aims of MHSR Journal

• To serve as an important medium for the publication of original research in the field of medical science and health research, thus filling gaps in health knowledge for effective utilization of research findings
• To impart current medical knowledge and updated scientific information obtained from research to health professionals for better and appropriate health care management
• To disseminate recent basic, applied and social research findings among health personnel of different strata for enhancing worldwide health development

Archives   2008

Myanmar   Health   Sciences   Research   Journal

Volume   20,  Number   2

TITLE:   Dot blot hybridization method for rapid detection of drug resistant Mycobacterium leprae in Myanmar.
AUTHOR:   Khin San Aye; Aye Aye Win; Khin Than Maw; Kyaw Kyaw; Masanori Matsuoka; Yasuhiko Suzuki
SOURCE:   Myanmar Health Sciences Research Journal. 2008; 20(2): 67-70
ABSTRACT:   Information of susceptibility for the Mycobacterium leprae isolates is beneficial for the proven multi-drug treatment and for verification of the efficacy of current leprosy control program. The simple genotypic method to detect mutations conferring resistance to dapsone (DDS), rifampin, and quinolone was exploited on the basis of hybridization with capture probe fixed to the glass slide. Mutations were discriminated by a series of oligonucleotide probes corresponding to each mutation in the folP, rpoB, and gyrA genes of Mycobacterium leprae. Based on these mutations, gene contained these mutation points (hot spots) was amplified by polymerase chain reaction (PCR) and followed by dot blot hybridization to detect mutations. This method is simple, rapid and not very expensive. The result can be obtained within 8 hours. A total of 100 mutibacillary leprosy cases attending the Central Special Skin Clinic, Yangon General Hospital were determined gene mutations. Ninety-three cases (93%) were susceptible to three drugs and seven cases (7%) were resistant. Among resistant cases, 3 were DDS resistance, 1 was rifampicin resistance, 2 were quinolone resistance and 1 was both DSS and rifampicin resistance (MDR).
SUBJECT HEADINGS:   Hybridization. Drug Resistance. Mycobacterium leprae. Immunoblotting.
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