Myanmar Health Sciences Research Journal
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Aims of MHSR Journal
  • To serve as an important medium for the publication of original research in the field of medical science and health research, thus filling gaps in health knowledge for effective utilization of research findings
  • To impart current medical knowledge and updated scientific information obtained from research to health professionals for better and appropriate health care management
  • To disseminate recent basic, applied and social research findings among health personnel of different strata for enhancing worldwide health development


Archives   2008

Myanmar   Health   Sciences   Research   Journal

Volume   20,  Number   1

TITLE:   Diarrhoeagenic Escherichia coli from childhood diarrhea.
AUTHOR:   Myat Thidar; Phyu Win Ei; Mi Mi Htwe; Aye Aye Maw; Wah Wah Aung
SOURCE:   Myanmar Health Sciences Research Journal. 2008; 20(1): 22-26
ABSTRACT:   A cross-sectional study was done to characterize the diarrhoeagenic Escherichia coli (DEC), isolated from stool samples of children under 5 years of age with diarrhoea who were admitted to the Paediatric Ward of North Okkalapa General Hospital from January to September, 2007. DEC was isolated from 16 stool samples among 60 samples collected. Char-acterization of DEC was done by serotyping, toxin assay and polymerase chain reaction (PCR). Serotyping identified 8 enteropathogenic E. coli (EPEC), 3 enterotoxigenic E. coli (ETEC), and one enteroinvasive E. coli (EIEC). Four isolates were not serotypable. The primers used in PCR assay were LTI-O1 and LTI-O2 for ETEC-Labile Toxin; ST-157 and ST-158 for ETEC-Stable Toxin; STX-O1, STX-O2, STX-101 and STX-102 for enterohaemorrhagic E. coli (EHEC). PCR assay result showed 3 EHEC and one ETEC. Latex agglutination test was done for verotoxin (VT) and heat-labile toxin (LT) and ELISA test for heat-stable toxin (ST). Toxin assays showed 4 VT, 4 ST and one with both VT and ST. In this study, serology results did not correspond to toxin assay and PCR results indicating that serotyping alone is not sufficient for diagnosis of pathogenic type for DEC.
SUBJECT HEADINGS:   Diarrhea. Escherichia coli.
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Vision : Achieving a healthier nation through application of research findings          Mission Statement : To Develop and promote solutions to the major health problems of Myanmar