Myanmar Health Sciences Research Journal
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Aims of MHSR Journal
  • To serve as an important medium for the publication of original research in the field of medical science and health research, thus filling gaps in health knowledge for effective utilization of research findings
  • To impart current medical knowledge and updated scientific information obtained from research to health professionals for better and appropriate health care management
  • To disseminate recent basic, applied and social research findings among health personnel of different strata for enhancing worldwide health development


Archives   2009

Myanmar   Health   Sciences   Research   Journal

Volume   21,  Number   3

TITLE:   Development of cytogenetic method for identification of Anopheles culicifacies species complex and its applications at malaria endemic areas of Myanmar.
AUTHOR:   Maung Maung Mya; Pe Than Htun; Sein Min; Sein Thaung
SOURCE:   Myanmar Health Sciences Research Journal. 2009; 21(3): 157-163
ABSTRACT:   Cytogenetic method for detailed identification of An. culicifacies was developed and tested in the field and laboratory. The objective of the study was to develop polytene chromosome cytogenetic method for An. culicifacies and to identify its sibling species complex by ovarian nurse cell polytene chromosome technique in malaria endemic areas of Myanmar. The study was conducted in two endemic areas, Paukkaung Township, Bago Division and Madaya Township, Mandalay Division. Adult mosquitoes were collected by animal baited big net trap and WHO recommended sucking tube in the field. Blood fed An. culicifacies were kept for 12 hours, 24 hours and 36 hours and ovaries were dissected and fixed in 10% methyl alcohol and ethyl alcohol fixatives in the field. In main laboratory, they were kept at 4ËšC in refrigerator. Ovarian nurse cell chromosome bands were examined by compound microscope under 400x magnifications. Blood fed An. culicifacies accounting 613 specimens from Bago Division and 35 specimens from Mandalay Division were examined and the result revealed that both methyl alcohol and ethyl alcohol were found to be suitable fixatives and preservatives for prolong period. Up to 70% to 80% of clear and accurate polytene chromosome bands were detected from the 36 hours aged ovary group. Furthermore, sibling species of An. culicifacies were studied in detail by using this cytogenetic method and found to be type "B" of An. culicifacies complex. It is assumed that in Myanmar type B species are abundant in Bago and Mandalay Divisions. However, further study is needed to find out the presence of other type of species complexes in endemic areas. Although An. culicifacies is not a primary vector in Myanmar, it has been abundantly present in association with primary vectors such as An. dirus and An. minimus in endemic areas.Better understanding of species complex distribution and the role in malaria transmission will be beneficial in prevention and control of malaria.
SUBJECT HEADINGS:   Malaria. Anopheles. Cytogenetics.
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Vision : Achieving a healthier nation through application of research findings          Mission Statement : To Develop and promote solutions to the major health problems of Myanmar