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Original Articles 2020-09-11

Is Community Intervention Effective for Improving Maternal, Newborn and Child Health Care in Hard-to-Reach Areas of Chin State, Myanmar?

To tackle the maternal, newborn and child health (MNCH) care needs is a challenging situation in remote and hard-to-reach areas. Intervention activities focusing on strengthening community health care network through community participation and supporting Basic Health Staffs for MNCH ser

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Incidence of Snakebite in Four Townships of Yangon Region

The true incidence of snakebite in Myanmar is unknown. The current data are based on hospital returns to Ministry of Health and Sports and they are likely to be under-reported. A community-based survey is useful to know the current burden of snakebite and thus for planning and formulating str

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Aims of MHSR Journal

• To serve as an important medium for the publication of original research in the field of medical science and health research, thus filling gaps in health knowledge for effective utilization of research findings
• To impart current medical knowledge and updated scientific information obtained from research to health professionals for better and appropriate health care management
• To disseminate recent basic, applied and social research findings among health personnel of different strata for enhancing worldwide health development

Archives   2009

Myanmar   Health   Sciences   Research   Journal

Volume   21,  Number   3

TITLE:   Cloning of the hepatitis B surface antigen gene (2): amplification, restriction enzyme digestion and ligation of HBsAg gene and yeast plasmid pPIC9K.
AUTHOR:   Win Aung; Yeon Hee Kim; Hyunah-Kim; Suk Hoon Ha; Khin Pyone Kyi
SOURCE:   Myanmar Health Sciences Research Journal. 2009; 21(3): 126-131
ABSTRACT:   DNA cloning involves separating a specific gene or DNA segment from a larger chromosome, attaching it to a small molecule of carrier DNA, and then replicating this modified DNA thousands or millions oftimes through both the increase in cell number and the creation of multiple copies of the cloned DNA in each cell. The result is selective amplification of a particulargene or DNA segment. In a recent preliminary study, extraction and purification of the HBsAg gene from genomic DNA of H. polymorpha transformant cell, followed by ligation with pGEM-T vector and transfor-mation into the competent E. coli DH5 alpha cells were successfully carried out. In this study, the HBsAg gene containing plasmid pGEM-T was amplified by using specific primers with restriction enzyme (RE) digestable nucleotide sequences followed by RE digestion with EcoR I and Not I to obtain sticky ends for an effective ligation. At the same time, the yeast expression plasmid, pPIC9K was digested with same RE to have sticky ends compatible with those of HBsAg gene containing plasmid. Then, these DNA segments with compatible nucleotide sequences were ligated, followed by successful transformation into the competent E. coli cells. The results from each and every step were confirmed by direct PCR identification, RE analysis followed by agarose gel electrophoresis determination and DNA sequencing analysis. These initial steps are important and essential for furthertransformation of the recombinant HBsAg gene into thehostPischia pastoris yeast cell which can be used as a more productive organism for production of recombinant hepatitis B vaccine in the near future.
SUBJECT HEADINGS:   Hepatitis B Surface Antigen. Ligation. Digestion. Yeasts. Plasmids.
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