Sub acute Toxicity and Analgesic Activity of Wei Dha Jaza: Hsei: Pale: Kala´ (TMF-23) on Animal Models

Moh Moh Aung1*, Thandar Khaing1, Chan Mya Thu1, Khin Taryar Myint1, Moh Moh Htun2, Htay Htay Kywal2, Sandar Lin2 & Thein Zaw Linn1

Myanmar Health Sciences Research Journal, Vol. 37, No. 1-3, 2025

Wei Dha Jaza. Hsei: Pale: Kala´(Traditional Medicine Formulation-23, TMF-23) is one of the most useful drugs which is prescribed in tradi- tional clinics and hospitals throughout Myanmar. It has been used traditionally since 1907. It is remarked as Myanmar National Intangible Cultural Heritage in 2020. Myanmar traditional practitioners mainly prescribed TMF-23 as an analgesic and anti-inflammatory drug. This study was done to determine sub-acute toxicity and analgesic activity of Wei Dha Jaza. Hsei: Pale: Kala´ (TMF-23) on animal models. Sub-acute toxicity study of three doses (200 mg/kg body weight, 400 mg/kg and 600 mg/kg) of TMF-23 was conducted on albino rats according to the Organization for Economic Cooperation and Development (OECD) guideline 407. Analgesic activities of three difference doses of TMF-23 (400 mg/kg, 800 mg/kg and 1200 mg/kg) and standard drug, Diclofenac sodium (50 mg/kg) were done by acetic acid induced writhing test on albino mice. In Subacute toxicity, there were no gross abnormalities in all organs except brown color changes in small intestine in one rat of each group. Microscopically, some intestinal glands had dysplastic changes in small intestine of one rat in each group. 

Wei Dha Jaza. Hsei: Pale: Kala´ (ဝေသာရဇ္ဇ
ဆေးပုလဲကလာပ်) (TMF-23) is described in
Dhatu-vi-par-ka-dipani (ဓာတုဝိဘာဂဒီပနီကျမ်း) and

it has  been used traditionally since 1907.¹
It is remarked as Myanmar National Intangible

Cultural Heritage in 2020.² TMF-23 composed of twenty-four plants origin and one
chemical origin which has hot, bitter
and pungent taste. 

Ingredients of Wei Dha Jaza. Hsei: Pale: Kala´ (TMF-23)

The ingredients of the formulation of
TMF-23 are shown in Table 1.⁶ The raw materials for TMF-23 were collected from Zegyo Market, Mandalay. These raw  

TMF-23 is potent analgesic drug used by majority of the Traditional Medicine Practitioners. It is widely prescribed analgesic drug for various types of pain at Traditional Medicine Hospitals and clinics. Sub-acute
toxicity study of three doses (200 mg/kg, 400 mg/kg and 600 mg/kg) of TMF-23 was conducted in 24 albino rats according to OECD guideline 407 and analgesic activity of three difference doses of TMF-23
(400 mg/kg, 800 mg/kg and 1200 mg/kg) was done by acetic acid induced writhing test on 30 albino mice. 

Firstly, we would like to express our gratitude to Implementation Research Grant Committee for financial support for research work. We would like to give our heartily thanks to all members of the Ethical Review Committee of University of Traditional Medicine for the acceptance of this protocol and for allowing us to conduct this study. We are also grateful to Dr. Khin Moe Aung, Mandalay General Hospital for her professional help for this study. We are deeply thankful to our colleagues and co-investigators for their supporting throughout the study.

There were no competing interests.

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of Traditional Medicine Formulations
(TMF-6 & TMF-23). Myanmar Health Science Research Journal 2014; 16(1-3):18-22.

2.       May Aye Than, Mu Mu Sein Myint,
Khin Myo Naung, Aye Than, Myint Myint Khine, San San Myint, et al. Anti-inflammatory activity of essential oil from some plant ingredients of traditional medicine formulation. Programme and abstracts of Myanmar Health Research Congress 2014; Yangon, Myanmar. p.13.

3.       Bhat Mohd Skinder., Bashir Ahmad Ganai, & Abdul Hamid Wani. Scientific Study of Gentiana kurroo Royle (MDPI). Medicines 2017; 4(4): 74.