Myanmar Health Sciences Research Journal
Original Articles :
Myanamr Health Research Registration 2023; 35(1-3): 34-41.
DOI:

Acute Toxicity and Hypoglycemic Activity of Dregea volubilis Benth. Leaves (ေခွးေတာက်) on Animal Model

Khine Khine Lwin*, Khin Phyu Phyu, Phyu Phyu Win, Aye win Oo, Nu Nu Win, Myint Myint Khine, San San Myint and Mie Mie Thaw

Myanmar Health Sciences Research Journal, 2023; 35(1-3):34-41

ABSTRACT

Dregea volubilis Benth. is traditionally used for treatment of Diabetes Mellitus in India and Myanmar traditional medicines. This study was performed to determine phytochemical constituents, acute toxicity in mice and hypoglycemic activity of 70% ethanolic extract of Dregea volubilus Benth. leaves in alloxan induced diabetic rats. Phytochemical constituents of the leaves were investigated by using the methods of Harborne (1984) and Physicochemical standards of Unani Formulations (1987). Acute toxicity study of the extract of the leaves in mice was done according to Organization for Economic Co-operation and Development guideline (OECD 423). Hypoglycemic activity was determined in alloxan induced diabetic rats. Hyperglycemia was induced in adult healthy albino rats of both sexes by single intraperitoneal injection of 5% alloxan monohydrate (120 mg/kg).


RESULT
Goto

INTRODUCTION
Goto

Diabetes mellitus is a chronic disease and it is one of the common metabolic disorders with micro and macro vascular compli-cations that results in significant morbidity and  mortality.  Diabetes  is  a  major cause of blindness, renal failure, heart attacks, stroke and lower limb amputation. World Health Organization (WHO) reported that  the number  of  people with Diabetes mellitus was 422 million in 2014. Diabetes prevalence has been rising more rapidly in low- and middle-income countries than in high-income countries. WHO estimates that diabetes was the seventh leading cause of death in 2016.1


SUPPLEMENTARY MATERIAL
Goto

Study design

Study design was Laboratory based experimental study.

Place of the study

Place of the Study was in Pharmacology Research Division, Department of Medical Research.

Collection and extraction of leaves

Dregea volubilis Benth. mature leaveswere purchased from Thiri Mingalar market in Yangon and was identified by a Botanist from Botany Department, Yangon University. The leaves were cut into small pieces and air dried in the shade. Air dried leaves were made into powder by using grinding machine. Hundred grams of the dried powder of leaves were put into 5 L conical flask and 1 L of 70% ethanol was added. The flask was put on 60°C water bath for 6 hours for extraction. Then, mit was cooled down to room temperature. The mixture was filtered through filter paper and the filtrate was evaporated by using water bath at 100°C to get dried extract.12


DISCUSSION
Goto

It was reported that acute oral toxicity studies of ethanol extract of Dregea volubilis Benth. leaves in albino mice showed that the extract was not toxic upto the dose of 2g/kg b.w in albino mice.19 In this study, acute toxicity study of 70% ethanolic extract of Dregea volubilis Benth. leaves showed that the extract had no toxic effect and lethality in mice up to maximum dose of 5 g/kg b.w. Therefore, LD50 of the extract was more than 5g/kg b.w.

Alloxan is a cyclic urea compound which induces permanent diabetes mellitus Administration of alloxan cause rapid destruction of β islet cells of pancreas and cause   cell   death.  


ACKNOWLEDGMENT
Goto

Authors are thankful to Director General, Dr Zaw Than Tun (Department of Medical Research) for allowing us to perform this research.


CONFLICT OF INTEREST
Goto

REFERENCES
Goto

1      World Health Organization, Geneva. Diabetes [Internet] (8 June 2020) Available from: https:// www.who.int/news-room/ fact-sheets/detail/diabetes

2.     Saeedi P, Petersohn I, Salpea P, Malanda B, Karuranga S, Unwin N, et.al. Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045: Results from the International Diabetes Federation Diabetes Atlas, 9th ed. Diabetes Research and Clinical Practice 2019; 157: 107843.  [Internet]  Available from: https:// www. diabetesresearchclinicalpractice. com/ article/ S0168-8227(19)31230-6/ fulltext.

3.     Mya Bwin & Sein Gwan. Plants with Reputed hypoglycemic action. In: Burmese Indigenous Medicinal Plants. Rangoon, Burma Medical Research Institute, 1967; Special report series; (4).